Enclosure (1) to COMDTINST 6510.1
NOTE: WHEN COLLECTING BLOOD ON THE BLOODSTAIN CARD,
ENSURE THAT YOU DO NOT CONTAMINATE THE CARD WITH
BLOOD FROM ANOTHER INDIVIDUAL. THIS WILL INTERFERE
WITH THE DNA IDENTIFICATION. THEREFORE, ENSURE THAT ALL
WORK SURFACE AREAS, GLOVES AND OTHER SUPPLIES ARE
CLEAN AND FREE OF BLOOD AND OTHER BODY FLUIDS BEFORE
PROCEEDING WITH ANOTHER COLLECTION.
(b)
Venipuncture Method: Prior to venipuncture, ensure that the vacutainer tube
contains the name and SSN identifiers specific to the service member,
deploying civilian or contractor who is providing the blood specimen. Using
standard sterile venipuncture techniques, draw the blood into a purple top
vacutainer tube in which contains only ethylenediaminetetraacetic acid
(EDTA). No other type of vacutainer shall be used. The DNA Specimen
Repository offers a 6" X 8" transfer pouch that can be used to keep an
individual's bloodstain card, oral swab vial, and vacutainer of blood together.
The use of this pouch will facilitate keeping a service member's collection
material together until it can be spotted. If a collection site uses the 6" X 8"
pouch it will expedite the collection process, and allow collection personnel to
return individuals to duty faster, and the collection personnel to spot the
bloodstain cards in a less cluttered location. To transfer blood from the
vacutainer tube to the bloodstain card, place the bloodstain card such that the
filter paper side containing the printed circles is facing up. Taking precautions
to prevent splatter or surface contamination, remove the top of the vacutainer.
For personal protection, use laboratory blood spatter shields if available.
Using a standard plastic disposable transfer pipette, place four drops of blood
in the center of each of the four circled areas on the bloodstain card. Allow
the blood to migrate throughout the circle to completely fill the circled area.
If required, additional blood droplets may be applied to the circle. It is
acceptable to have blood outside the designated circles. The more blood the
better. Once the blood transfer is completed, dispose of the remaining blood,
vacutainer, and pipette in an approved container. Do not reuse the plastic
transfer pipette.
(c)
Drying of the Bloodstain Card: After affixing the blood to the bloodstain card,
allow the blood to dry using the drying racks furnished by the DNA Specimen
Registry. NOTE: DO NOT STACK THE BLOODSTAIN CARDS OR
TOUCH THE BLOOD STAIN AREAS OF THE CARD WHILE
TRANSFERRING THE BLOODSTAIN CARDS TO THE DRYING
RACKS. ENSURE THAT GLOVES AND ALL SURFACE AREAS OF
THE DRYING RACK ARE CLEAN, AND FREE OF BLOOD OR OTHER
BODY FLUIDS. IN PLACING THE BLOODSTAINED CARDS INTO THE
DRYING RACK, ENSURE THAT THE WET BLOOD STAINED ENDS
DO NOT COME IN CONTACT WITH OTHER CARDS. THIS CAN BE
ACCOMPLISHED BY
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